Isolation and characterization of protein A24, a "histone-like" non-histone chromosomal protein.

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Isolation and characterization of protein A24, a "histone-like" non-histone chromosomal protein.

In earlier studies, the nucleolar levels of protein A24 were found to be markedly decreased in the nucleolar hypertrophy induced by thioacetamide or during liver regeneration (Ballal, N.R., Goldknopf, I.L., Goldberg, D.A., and Busch, H. (1974) Life Sci. 14, 1835-1845; Ballal, N.R., Kang, Y.-J., Olson, M.O.-J., and Busch, H.J. Biol. Chem. 250, 5921-5925). To determine the role of protein A24, me...

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Isolation and Characterization of Protein A24, a “Hi&one-like” Non-Histone Chromosomal Protein*

In earlier studies, the nucleolar levels of protein A24 were found to be markedly decreased in the nucleolar hypertrophy induced by thioacetamide or during liver regeneration (Ballal, N. R., Goldknopf, I. L., Goldberg, D. A., and Busch, H. (1974) Life Sci. 14, 1835-1845; Ballal, N. R., Kang, Y.-J., Olson, M. O.-J., and Busch, H. J. Biol. Chem. 250, 5921-5925). To determine the role of protein A...

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Tissue-specific Chromosomal Non-histone Protein Interactions

A group of chromosomal non-histone proteins with affinity for DNA (NP) was isolated from rat liver and Novikoff hepatoma. This fraction, which represents less than 5% of the total chromatin protein content, binds preferentially to unique, double-stranded sequences of fractionated homologous DNA. The interactions are strong at low ionic strength (K, = 6.7 x lOme M) and decrease with rising salt ...

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Characterization of a histone-like protein extracted from yeast mitochondria.

Analysis of proteins isolated by affinity chromatography on DNA-cellulose from highly purified yeast mitochondria shows that these organelles do not contain histones but have in abundance a DNA-binding protein of 20,000 daltons. The purification yield of this protein, called HM, indicates that mitochondria have at least an equal mass of HM relative to DNA. The amino acid composition and its ele...

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Tissue-specific chromosomal non-histone protein interactions with DNA.

A group of chromosomal non-histone proteins with affinity for DNA (NP) was isolated from rat liver and Novikoff hepatoma. This fraction, which represents less than 5% of the total chromatin protein content, binds preferentially to unique, double-stranded sequences of fractionated homologous DNA. The interactions are strong at low ionic strength (Km = 6.7 X 10(-9) M) and decrease with rising sal...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1975

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)40926-5